The trade-off between quality and quantity is a fundamental economic dilemma. Now, a team of British, American, and Japanese researchers describes how it applies to biology, as well. They have discovered that this dilemma most likely shaped the evolutionary trajectory of ants, one of Earth’s most successful groups of organisms.
Their study reveals that, as ant societies grew in complexity and numbers, they didn’t just make their workers smaller—they also made them cheaper.
The cost of armor
In the insect world, the exoskeleton known as the cuticle serves as a protective barrier against predators, pathogens, and desiccation, while providing the structural framework for muscle attachment. But this protection comes at a price. Building a robust cuticle requires significant amounts of nitrogen and rare minerals like zinc and manganese. While skimping on armor for an individual insect may be a death sentence, the evolution of ants apparently found a way around it.
“There’s this question in biology of what happens to individuals as societies they are in get more complex?” said Evan Economo, an entomologist at the University of Maryland and co-author of the study. “For example, the individuals may themselves become simpler because tasks that a solitary organism would need to complete can be handled by a collective.”
Economo’s team hypothesized that the metabolic balance behind investing in cuticles in social insects like ants could favor the collective over the individual. The idea was that a colony of 10,000 workers could lose a few individuals to a predator without much consequence, so investing heavily in each worker’s defenses would seem like a waste of precious nutrients. To test this hypothesis, they examined whether ant lineages that maintain massive, specialized workforces reduce the investment in their individual workers’ exoskeletons.
Scanning superorganisms
To test this idea, the researchers needed to pull off a comparative study on the anatomy of ants at an unprecedented scale. “We worked with scans of ant specimens and species from all over the world to capture the global diversity of ants,” Economo says. The team used a massive database called Antscan, which contains three-dimensional X-ray microtomography imaging of ants from around the globe.
Microtomography works similarly to medical CT scans, but at a vastly higher resolution. Still, on its own, the technique could only generate lots of precise data—it still had to be interpreted. Parsing through 3D imagery of over 880 specimens, including workers, queens, and males belonging to over 500 different species, was another challenge. “The 3D scanning itself is a very advanced technology,” says Arthur Matte, a researcher at the University of Cambridge and lead author of the study. “But once you have the ants in three dimensions, it’s still very hard to segment manually every tissue you’re interested in.”
To solve this, Matte developed a computer vision algorithm for “unsupervised segmentation.” Because the cuticle is always the outermost tissue of an arthropod, the algorithm could automatically identify and measure the volume of the exoskeleton across all ants in the dataset.
The first thing scientists noticed when the results poured in was that cuticle investment varied wildly, ranging from 6 percent to 35 percent of an ant’s total body volume. So, the next thing they focused on was figuring out the reasons behind these variations.
The numbers game
The team started checking how factors like diet, temperature, humidity, and foraging style correlated with the size of the cuticle. To get a handle on this, segmented 3D scans were fed into evolutionary models. “One of the most insightful things we did was to individually remove such variables from the models to estimate their contributions to the final cuticle investment,” Matte explains. This way, scientists learned that temperature was responsible for just 12 percent of variation in cuticle size and diet—especially its nitrogen content—explained another 37 percent. But the factor that had the strongest impact on the model was the colony size.
Ants that invested less in their cuticle tended to have significantly larger colony sizes. More surprisingly, this reduction in cuticle investment and the resulting increase in colony size appeared to be associated with higher diversification rates. In biological terms, squishier ants could evolve to occupy new niches much faster than their heavily armored cousins. “Requiring less nitrogen could make these ants more versatile and able to conquer new environments,” Matte suggests. This efficiency may have allowed ants to transition from a diet of high-protein prey to more abundant but less nutritious liquid sugar sources, like honeydew or floral nectar.
“Ants reduce per-worker investment in one of the most nutritionally expensive tissues for the good of the collective,” Matte explains. “They’re shifting from self-investment toward a distributed workforce.”
Power of the collective
The researchers think the pattern they observed in ants reflects a more universal trend in the evolution of societal complexity. The transition from solitary life to complex societies echoes the transition from single-celled organisms to multicellular ones.
In a single-celled organism, a cell must be a “jack-of-all-trades,” performing every function necessary for survival. In a multicellular animal, however, individual cells often become simpler and more specialized, relying on the collective for protection and resources.
“It’s a pattern that echoes the evolution of multicellularity, where cooperative units can be individually simpler than a solitary cell, yet collectively capable of far greater complexity,” says Matte. Still, the question of whether underinvesting in individuals to boost the collective makes sense for creatures other than ants remains open, and it most likely isn’t as much about nutritional economics as it is about sex.
Expendable servants
The study focused on ants that already have a reproductive division of labor, one where workers do not reproduce. This social structure is likely the key prerequisite for the cheap worker strategy. For the team, this is the reason we haven’t, at least so far, found similar evolutionary patterns in more complex social organisms like wolves, which live in packs—or humans with their amazingly complex societies. Wolves and people are both social, but maintain a high degree of individual self-interest regarding reproduction. Ant workers could be made expendable because they don’t pass their own genes—they are essentially extensions of the queen’s reproductive strategy.
Before looking for signs of ant-like approaches to quality versus quantity dilemmas in other species, the team wants to take an even closer look at ants. Economo, Matte, and their colleagues seek to expand their analysis to other ant tissues, such as the nervous system and muscles, to see if the cheapening of individuals extends beyond the exoskeleton. They are also looking at ant genomes to see what genetic innovations allowed for the shift from quality to quantity. “We still need a lot of work to understand ants’ evolution,” Matte says.
Science Advances. 2025. DOI: 10.1126/sciadv.adx8068














